 The
detection of illegal drugs has been an established procedure
in forensic toxicology since the mid-1990s. Once ingested,
opiates, cocaine, cannabinoides and amphetamines are metabolized
in the body and their breakdown products enter permanently
into the hair root from where they are deposited permanently
in the hair in characteristic relationships parent substance/metabolite.
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FAEE vs.
Egt Hair Testing
FAEEs, measured
in nanogram quantities (1 ng = one billionth of a gram)
appear in hair in almost one order of magnitude higher
than the relevant order of magnitude of Etg, measured
in picogram quantities (1 picogram = one trillionth
of a gram).
It has been technically
possible to measure FAEEs since 1993, whereas the technique
for measuring the relevant range of Etg is still in
its infancy.
In practice, it
is likely that some hair which is sent for analysis
has been cosmetically treated in some way (bleached
or permed etc.). It has been proven that FAEEs are not
significantly affected by such treatments. So far no
systematic investigations in this regard have been carried
out for Etg.
FAEE concentrations
in hair from other body sites can be interpretated in
a similar fashion as scalp hair. For Etg there is currently
no information available.
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As hair grows from the root (at approximately
1 cm per month), these deposits are delayed to a certain extent,
therefore, they can not be detected until the hair grows out
a bit (2-4 weeks). The distance from the scalp of the hair
portion containing drugs allows us to estimate the approximate
time of drug consumption. The deposited substances are relatively
stable in the face of external influences such as hair treatments,
cosmetics and sunlight. Due to the way in which drugs are
deposited in the hair, hair analysis provides us with a detailed
view of drug consumption over the past weeks or months. Consumption
on a day-to-day basis can be detected by analyzing blood and
urine samples.
Trace amounts of ethanol from the environment are present
in all hair including in the hair of teetotalers. Simply being
in a pub or laboratory is enough for traces of ethanol to
find their way into the hair.
In contrast to other drugs consumed, alcohol is not deposited
directly in the hair. For this reason the investigation procedure
looks for direct products of ethanol metabolism. The main
part of alcohol is oxidized in the human body. This means
it is released as water and carbon dioxide. One part of the
alcohol reacts with fatty acids to produce esters. The sum
of the concentrations of four of these fatty acid ethyl esters
(FAEEs: ethyl myristate, ethyl palmitate, ethyl oleate and
ethyl stearate) are used as indicators of the alcohol consumption.
The amounts found in hair are measured in nanograms (one nanogram
equals only one billionth of a gram), however with the benefit
of modern technology, it is possible to detect such small
amounts.
In the mass spectrometer the substances are fired with an
electronic beam. Every molecule decomposes into specific fragments.
It is possible to determine which substance is present on
basis of its molecular weight.
However there is one major difference between most drugs
and alcohol metabolites (FAEE) in the way in which they enter
into the hair: on the one hand like other drugs FAEEs enter
into the hair via the ceratinocytes, the cells responsible
for hair growth. These cells form the hair in the root and
then grow through the skin surface taking any substances with
them. On the other hand the sebaceous glands produce FAEEs
in the scalp and these migrate together with the sebum along
the hair shaft (Auwärter et al., 2001, Pragst et al.,
2004). So these glands lubricate not only the part of the
hair that is just growing at 0.3 millimeters per day on the
skin surface, but also the more mature hair growth, providing
it with a protective layer of fat.
 Fig
1: Possible ways of incorporation of FAEEs into hair (based
on Auwärter, 2006).
This means the glands supply the whole length of hair with
sebum, which in turn carries FAEEs into the hair, resulting
in an accumulative increase of FAEEs from proximal to distal
hair sections, see next figure (Auwärter et al., 2001).
This phenomenon has one important consequence: whilst most
other drugs are mainly stored in the hair via the root alone,
allowing toxicologists to establish time-resolved patterns
of consumption according to the length of hair provided, this
is not possible with alcohol (FAEEs) with respect to previous
drinking and abstinence. This would only be possible using
alcohol markers which enter the hair solely through the hair
root, but these have not yet been discovered.
However there exists a significant correlation between the
intensity of the alcohol intake and the concentrations of
the FAEEs in hair. Results between 0.05 and 30 ng/mg were
found in hair (Pragst et al., 2006).
Fig. 2: Profile of FAEE concentrations (based on
Pragst, 2004). Case 1 is a strictly abstinent person.
Case 2 is a social drinker. Case 3 is a patient in alcohol
rehabilitation (self-reporting 60 grams ethanol per day for
at least 6 months). The increase in FAEE concentrations from
proximal to distal sections up to a length of 5-10 cm found
in most cases could be explained by the sebum deposition route.
The hair is continuously bathed by sebum, and this leads to
an accumulation of the concentrations with increasing age,
i.e., distance of hair from skin.
As a consequence of the increasing FAEE concentrations from
proximal to distal it is preferable to always analyze, if
possible, a standard length of hair. For example analysis
of a 1cm hair sample taken close to the scalp will give a
much lower result than the analysis of a 6 cm long sample
also taken close to the scalp from the same person. For this
reason we standardly use the proximal 0-6 cm segment of head
hair in exactly the same procedure. For samples analyzed under
these standard conditions the cutoff level is set at 1.0 ng/mg.
The same holds true for body hair of any length (length is
genetically determined, body hair grows more slowly and has
different growth cycles, etc.). However for shorter head hair
the analysis changes (e.g.: for only 3 cm we reduce the cut
off level to 0.5 ng/mg). The closer the conditions (i.e. hair
length) are to the standard procedure, the more certain we
can be of the accuracy of the result.
A 6 cm long hair sample can be used to look back over 6 to
12 months (this is commonly used in Germany to test drivers).
If head hair is not available or is too short (minimum: 2
cm) underarm, chest, leg and pubic hair may be analyzed. Body
hair gives us a picture going back up to twelve months.
| AbuseCheck™
FAEE Hair Alcohol Test |
|
| |
Single participant FAEE Hair Alcohol Test, collection materials, laboratory
service, and laboratory report. Does not include Legal Specimen Collection
Package. (TAT w/shipping: 14 bus. Days) |
|
| |
$500.00 |
|
|
AbuseCheck™ Hair Drug Test (5-Panel) |
|
| |
Single participant 5-Panel Hair Drug Test, collection materials, laboratory
service, laboratory report. Does not include Legal Specimen Collection Package.
(TAT w/shipping: 10 bus. Days) |
|
| |
$100.00 |
|
|
AbuseCheck™ CDT Alcohol Blood Test |
|
| |
Single participant CDT Alcohol Blood Test, collection materials, laboratory
service, and laboratory report. Does not include Legal Specimen Collection
Package. (TAT w/shipping: 10 bus. Days) |
|
| |
$215.00 |
|
|
AbuseCheck™ EtG Hair Alcohol Test |
|
| |
Single participant EtG Hair Alcohol Test, collection
materials, laboratory service, and laboratory report. Does not include Legal
Specimen Collection Package. (TAT w/shipping: 14 bus. Days) |
|
| |
$250.00 |
|
|
AbuseCheck™ Hair Alcohol Test Combo (FAEE & EtG) |
| |
Single participant FAEE Hair Alcohol Test, EtG
Hair Alcohol Test, collection materials, laboratory service, and laboratory
report. Does not include Legal Specimen Collection Package. (TAT w/shipping:
14 bus. Days) |
|
| |
$695.00 |
|
|
AbuseCheck™ Alcohol Test Combo (FAEE, EtG, &
CDT) |
| |
Single participant FAEE Hair Alcohol Test, EtG
Hair Alcohol Test, CDT Alcohol Blood Test, collection materials, laboratory
service, and laboratory report. Does not include Legal Specimen Collection
Package. (TAT w/shipping: 14 bus. Days) |
|
| |
$839.00 |
|
|
AbuseCheck™ Hair Alcohol & Drug Test Combo (HDT + HAT) |
| |
Single participant Hair Alcohol Test (FAEE) and Hair Drug Test (5-Panel),
collection materials, laboratory service, and laboratory report. Does not
include Legal Specimen Collection Package. (TAT w/shipping: 14 bus. Days) |
|
| |
$600.00 |
|
| |
:: ADDITIONAL FEES :: |
|
|
Legal Specimen Collection Package |
|
| |
Locating and scheduling a legal collection site for a single participant/test,
establishing chain-of-custody for the samples, collections fees, and specimen
shipping. Used for FAEE, EtG, CDT, 5-Panel Drug
Testing. |
|
| |
$150.00 |
|
|
Hair Alcohol Test from Body Hair (Additional Fee) |
|
| |
Additional Fee for hair alcohol testing (either FAEE or EtG) from body hair if head hair is not available. |
|
| |
$100.00 |
|
|
LOD Hair Drug Test (Additional Fee) |
|
| |
Additional 5-Panel hair drug test option to analyze at the lowest level
of detection (most sensitive analysis). |
|
| |
$40.00 |
|
|
Expert Witness / Testimonial Fees |
|
| |
|
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Additional
Information:
Literature
Pragst F., Balikova M.A.: State of the art
in hair analysis for detection of drugs and alcohol
abuse; Clinica Chimic Acta 370 2006 17-49.
Auwärter V.: Fettsäureethylester
als Marker exzessiven Alkoholkonsums – Analytische Bestimmung
im Haar und in Hautoberflächenlipiden mittels Headspace-Festphasenmikroextraktion
und Gaschromatographie-Massenspektrometrie. Dissertation Humboldt-Universität
Berlin 2006.
Pragst F., Auwärter V., Kiessling B.,
Dyes C.: Wipe-test and patch-test ror alcohol misuse based
on the concentration ratio of fatty acid ethyl esters and
squalen CFAEE/CSQ in skin surface lipids. Forensic Sci Int
2004; 143:77-86.
Yegles M., Labarthe A., Auwärter V.,
et al.: Comparison of ethyl glucuronide and fatty acid ethyl
esters concentrations in hair of alcoholics, social drinkers
and teetotalers. Forensic Sci Int 2004; 145:167-173.
Madea B.,Mußhoff F.(Hrsg.): Haaranalystik
- Technik und Interpretation in Medizin und Recht. Deutscher
Ärzteverlag Köln 2004.
Hartwig S., Auwärter V. and Pragst F.:
Effect of hair care and hair cosmetics on the concentrations
of fatty acid ethyl esters in hair as markers of chronically
elevated alcohol consumption. Forensic Sci Int 2003a; 131:90-97.
Hartwig S., Auwärter V. and Pragst F.:
Fatty acid ethyl esters in scalp, pubic, axillary, beard and
body hair as markers for alcohol misuse. Alcohol Alcohol 2003b;
38,163-167.
Auwärter V.: Fattty acid ethyl esters
in hair as markers of alcoholconsumption. Segmental hair analysis
of alcoholics, social drinkers, and teetotalers. Clin Chem
2001; 47: 2114-2123.
Pragst F., Auwärter V., Sporkert F.,
Spiegel K.: Analysis of fatty acid ethyl esters in hair as
possible markers of chronically elevated alcohol consumption
by headspace solid-phase microextraction (HS-SPME) and gas
chromatography-mass spectrometry (GC-MS). Forensic Sci Int
2001; 121:76-88.
Spiegel K.: Untersuchungen zum Nachweis alkoholspezifisch
metabolisierter Substanzen aus menschlichen Haaren mit Gaschromatographie-Massenspektrometrie;
Dissertation Humboldt-Universität Berlin 1997.
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